DOUMANDJI Bahia

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Start date: 2013End date: 2016

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    THE INTERACTION OF L5 LARVAE OF LOCUSTA MIGRATORIA (LINNAEUS, 1758) (OEDIPODINAE, ACRIDIDAE) WITH BIOBESTICIDES
    (TRANS STELLARI, 2014) OUTTAR, FAHIMA; MAHDJOUBI, DJILLALI; BISSAD, FATMA; MOUHOUCHE, FAZIA; DOUMANDJI-MITICHE, Bahia
    Our work is based on the use of three biopesticides belonging to three different categories, a fungus Metarhizium anisopliae var acridum a IGRs Triflumuron (TFM) and an excerpt from the henna plant Lawsonia inermis, on L5 larvae of Locusta migratoria applied with the two modes of penetration, contact and ingestion. For this, we tested their effect on the morphology and mortality. The results show us that the three biopesticides involved morphological deformations and a total mortality of 100% in the L5 larvae.
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    Morphology and structure of adult male genitalia of Brachytrupes megacephalus Lefebvre, 1827 (Orthoptera, Gryllidae) in the southeast of Algeria
    (Journal of Entomology and Zoology Studies 2015; 3(6): 355-359, 2015) Lakhdari, Wassima; DOUMANDJI-MITICHE, Bahia; Acheuk, Fatma; Dehliz, Abderrahmene; Hammi, Hamida; M’lik, Randa; Soud, Adila; DOUMANDJI, Salaheddine
    Depending to the few and old data which have been conducted on the body morphology of Brachytrupes megacephalus Lefebvre, 1827 (Orthoptera, Gryllidae), we have investigated the structure of male genitalia in Algerian Sahara species to establish a basis for morphological study. The study was conducted in the laboratory of entomology in the experimental station of Sidi Mehdi. After isolation of the male genital apparatus, we find that its structure is consisted by the testis, accessory gland, ejaculatory duct and the phallic complex. The last organ is composed of epiphalus, guiding rod, ectoparamere, endoparamere, dorsal and median pouch. The spermatophore produced by Brachytrupes megacephalus was investigated in detail. Besides the study of the spermatophore size (8 mm), morphology, and formation, spermatophore is composed of the ampulla (3.9 mm), attachment plate (2.8 mm) and the tube (1.5 mm). The present paper aims to improve this information, on the species of B. megacephalus.
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    Acaricidal Activity of Aqueous Extracts against the mite of date palm Oligonychus afrasiaticus Meg (Acari: Tetranychidae)
    (Journal of Medicinal Plants Studies 2015; 3(6): 113-117, 2015) Lakhdari, Wassima; Dehliz, A; Acheuk, F; Soud, A; Hammi, H; Mlik, R; DOUMANDJI-MITICHE, Bahia
    The use of chemical products to fight against insects raises several concerns related to the environment, human health, non-target species and development of resistant pest populations. The use of bio-pesticides may reduce the risks of pests and safeguard biodiversity. This experiment was conducted with the objective to study the effect of aqueous plant extracts from the southeast region of Algeria (Zygophyllum album L, Cotula cinerea Del and Limoniatrum guyonianum Del) on the mortality of the date palm mite (Oligonychus afrasiaticus). The trial was conducted at the Laboratory of Plant Protection of INRAA. The five doses (1%, 2%, 3%, 4%, and 5%) were tested on adults of phytophagous. The results show that the extracts of plants and C. cinerae and L. guyonianum do not show an effect on the pest. Unlike, Z. album showed a very significant effect on the mite by a mortality rate of 76%.
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    Host Plant Determination of Brachytrupes megacephalus Lefebvre, 1827 (Orthoptera, Grillinae) Using Faeces Analysis in the Region of Oued Righ (Algerian Sahara)
    (American-Eurasian J. Agric. & Environ. Sci., 15 (2): 271-277, 2015, 2015) Lakhdari, Wassima; DOUMANDJI-MITICHE, Bahia; Dahliz, A; DOUMANDJI, Salaheddine; Bendifellah, L; Bouchikh, Y; Hammi, H; Soud, A; Mlik
    In the southeast of the algerian Sahara (Touggourt), the host plant determination of Brachytrupes megacephalus was studied by faeces analysis (microscopic observations). The experimentation was conducted in mid August until the end of September 2014. The main results of faeces analysis showed that the plants consumed by B. megacephalus belong to 8 families: Arecaceae (Phoenix dactylifera), Apocynaceae (Nerium oleander), Asteraceae (Chrysanthemum), Myoporaceae (Myoporum sandwicense), Plumbaginaceae (Armeria maritima), Rosaceae (Prunus armeniaca), Solanaceae (Solanum lycopersicum) and Triochylaceae (Cherry fantasia and White Kristina). Phoenix dactylifera is the most preferred host species by the adults of Brachytrupes megacephalus, with a consumption rate 85,87%.
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    Morphological Study of Different Developmental Stages of Brachytrupes megacephalus Lefebvre, 1827 (Orthoptera, Gryllidae) and Their Development in Oued Righ Region (Algerian Sahara)
    (journal international, 2015) Lakhdari, Wassima; DOUMANDJI-MITICHE, Bahia; Acheuk, Fatma; Dehliz, Abderrahmene; M’lik, Randa; Soud, Adila; Hammi, Hamida
    In the Southeast of the Algerian Sahara (Touggourt), a morphological study of Brachytrupes megacephalus was conducted during 2014. According to the damages caused by this cricket through deviation and loss of irrigation water due to deep tunnels dug and damage crops, we found it useful to identify the insect and study the external appearance of this cricket. The results show that B. megacephalus has an annual life cycle with five nymph stages. The mating season is placed normally from mid-March to mid-April. The eggs are with an ovoid shape and a greenish color measured by 3.5 to 4 mm, First nymph stage is with a transparent colors which has a pronotum measured between 1.5 and 2.2 mm with a head between 3 and 4,6 mm and the femur has a length of 5 to 6 mm. Unlike the other nymph stages are yellow-orange with a darker head than the rest of the body. The adults of this cricket have a pronotum of 5 to 6 mm, the head is between 13 to 14 mm and the femur is from 17 to 22 mm. All these post-embryonic development mature throughout the summer until September, during which the cricket becomes imago.
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    Diversity and Ecology of Orthoptera of Some Agricultural Areas in Northern Algeria
    (IJIAR, 2016) HAMADI, Kamel; DOUMANDJI-MITICHE, Bahia
    A study on the orthopterologic diversity was performed in three different agro-ecosystems in northern Algeria, located at Baba-Ali, Ain-Taya and Cap-Djinet. The first two stations are part of the plain of Mitidja. Inventories carried out were conducted over a period of 12 months; November-December 1996/1997 for Baba-Ali and Ain-Taya stations, and September-August 2010/2011 for Cap-Djinet station. The settlement of the identified orthopteric revealed a total of 25 species, 8 Ensifera and 17 Caelifera. Acrididae Family accounts only 15 species are spread over 6 subfamilies, 60% of the identified orthopterofauna. The highest total richness and the specific average richness per survey and station values were recorded in October 1997: 8 – 4, 2 Baba-Ali, 10-5,2 Ain-Taya; and in August 2011: 13 – 6,2 Cap-Djinet. According to the Shannon-Weaver diversity index, the surveyed areas have a diverse orthopterofauna particularly in spring and autumn when the number of individuals is equally distributed between species (0,62 < E < 0, 97).
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    ORTHOPTEROLOGIC FAUNA OF OUARGLA OASIS
    (TRANS STELLARI, 2014) BELHADJ, HAMIDA; DOUMANDJI-MITICHE, Bahia; GUENDOUZ-BENRIMA ATIKA, ATIKA
    Orthopterologic populating study has been conducted in 3 stations of Ouargla Oasis situated in south-west of Algeria. Orthopteras Samplings are realized every fortnight from September 2001 until August 2002. This inventory is completed by floristic statements for each station. 18 orthopteras species were inventoried with 16 Caeliferae where 14 belong to family of Acrididaes and so 2 species of Ensiferae belonging to Gryllidae family. Study of total and middle richness shows variation according to stations and to samplings periods. The most important yearly centesimal frequency is pronounced for Pyrgomorpha cognata specie in both palm groves, and for Acrotylus patruelis specie in the cultivated ground of Experimental farm. of Hassi Ben Abdullah. Constancy of Orthopterologic species varies from station to another. However, two species are constant for the three stations, which are Pyrgomorpha cognata and Ochrilidia gracilis. The most important diversity index is 1.66 bits in June month for Mekhadma station. Equitability values are all closer of 1. corresponding to populations in balance between them. The contagious distribution of individuals is the most characteristic. Through factorial analysis of correspondences, we have noted that orthopterologic species are spreading according to their ecologic affinities
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    ANTAGONISTIC ACTIVITY OF TWO BACILLUS SP. STRAINS ISOLATED FROM AN ALGERIAN SOIL TOWARDS THE MIGRATORY LOCUST LOCUSTA MIGRATORIA (LINNAEUS 1758)
    (2016) OULEBSIR-MOHAND KACI, Hakima; TALBI-KHEMILI, Souad; GANA-KEBBOUCHE, Salima; DOUMANDJI-MITICHE, Bahia
    The aim of our study is to isolate, purify and characterize two entomopathogenic bacterial strains of the genus Bacillus from the soil of Adrar in the Algerian Sahara. The molecular characterization of the isolated strains was carried out by DNA isolation, PCR and sequencing of 16S rRNA gene, followed by a phylogenetic analysis. After that, the study of the bacteria toxicity on the fifth larvae stage of Locusta migratoria was conducted by assessing rates of mortality, LT50 and LC50. The phylogenetic analysis showed that the two strains (Strain-B1 and Strain -B2) were identified as Bacillus sp. (HE799656) and Bacillus sp. (HE805963), respectively, with a very high similarity (99%) with the strains Bacillus thuringiensis (NR_043403) and Bacillus weihenstephanensis (NR_024697). Besides, the results of toxicity tests showed that larvae of L. migratoria are influenced by the two isolated bacterial strains. This action is more pronounced with the Bacillus sp. strain-B1 (HE799656) compared to Bacillus sp. strain-B2 (HE805963). Examination of TL50 shows that these times vary depending on the bacteria and concentrations applied. Similarly, the LC50 are closely related with time and they also vary depending on the bacterial strain tested.
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    Diversity and Richness of Rodent Communities in Various Landscapes of Touggourt Area (Southeast Algeria)
    (2015) Moussa, Hadjoudj; Karim, Souttou; Salaheddine, DOUMANDJI
    Our study presents the species diversity of rodent communities from palm groves and sand dunes in a desert area (Touggourt, Southeast Algeria). Samples were collected from four stations: two sites of sand dunes and tow sites of palm groves. The method of random trapping was performed using 24 cages of Besançon Technique Service (BTS) installed at each station. All captured species were of the family Muridae and belonged to two subfamilies: Murinae and Gerbillinae. The most common species at all stations was Gerbillus nanus (31 specimens), followed by Rattus rattus (23 specimens) and Gerbillus tarabuli (18 specimens). The murine species Rattus rattus and Mus spretus were found at agriculture sites (palm groves) while the Gerbillinae Gerbillus nanus, Gerbillus gerbillus and Gerbillus tarabuli preferred natural environments (sand dunes) where the soil was light and sandy.
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    A molecular phylogenetic and phylogeographic study of two forms of Calliptamus barbarus (Costa 1836) (Orthoptera: Acrididae, Calliptaminae) from two regions of Algeria
    (2016) Moad, ROUIBAH; Alejandro, López-López; Juan, José Presa; Salaheddine, DOUMANDJI
    Calliptamus barbarus (Orthoptera: Acrididae) is the most polymorphic species within the genus Calliptamus. It shows a morphological polymorphism (three hind femoral spots, or only one hind femoral spot). Several studies have been made in order to distinguish the two forms: morphometry, number of ovarioles, sound production, protein and enzyme system. The aim of our work is to assess whether the two forms can be considered as different taxa and to perform a molecular phylogenetic study of two populations of C. barbarus collected from two different Algerian localities. No clear genetic differentiation was found between the samples with different morphologies. Additionally, the samples from Algeria do not form a monophyletic sister clade compared to the one formed by the sequences from GenBank from other geographical regions. Despite the morphological differences shown between the two populations, our molecular study indicates that there are no differences at a molecular level using the two mitochondrial genes COI and 16S.